抗体人源化

• samples	Chi² (RU²)	ka (1/Ms)	kd(1/s)	KD (M)	Rmax (RU)
  humanized antibody	5.03E-02	3.67E+05	6.90E-05	1.88E-10	70.5
  parental antibody	2.11E-01	4.02E+05	5.56E-05	1.38E-10	73.6

  Table.1 Affinity kinetic analysis

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  Fig.1 Affinity kinetic analysis of humanized antibody

• 人源化抗体和亲本抗体ELISA检测结合活性如图所示，在抗体人源化改造前后EC50值，上下平台值高度相似。

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  Fig.2 Comparison of the affinity of humanized antibodies to parental antibody

• 人源化抗体和亲本抗体FACS检测结合活性如图所示，在抗体人源化改造前后EC50值，上下平台值高度相似。

• 

  Fig.3 Comparison of the affinity of humanized antibodies to parental antibody

“We are pleased with the antibody humanization service and the purification service of ProBio. It is very impressive and professional. we validated the humanization service by testing the antibody against anti-human IgG as the secondary antibody. The humanized antibody purification met our expectations and need. The characterization of the antibody that was highlighted by the COA is excellent. We would continue to avail your service and will also recommend it to anyone who needs such service. Thanks.” – Govind Panchamoorthy, Director, Genus Oncology