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Construction and Immunogenicity Evaluation of Recombinant Influenza A Viruses Containing Chimeric Hemagglutinin Genes Derived from Genetically Divergent Influenza A H1N1 Subtype Viruses.

PLoS ONE. 2015; 
McCormickKara,JiangZhiyong,ZhuLongchao,LawsonSteven R,LangenhorstRobert,RansburghRussell,BrunickColin,TracyMiranda C,HurtigHeather R,MabeeLeah M,MingoMark,LiYanhua,WebbyRichard J,HuberVictor C,Fang
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Codon Optimization … ELRSRYWAIRTRSG). After being quadruplicated and linked with linkers (-KK- for HA91-108 and M2e, -RVKR- for NP366-374 and NP380-393), the genes were codon-optimized to mice and synthesized by GenScript (Piscataway, NJ) … Get A Quote

摘要

Influenza A viruses cause highly contagious diseases in a variety of hosts, including humans and pigs. To develop a vaccine that can be broadly effective against genetically divergent strains of the virus, in this study we employed molecular breeding (DNA shuffling) technology to create a panel of chimeric HA genes.

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