| Products/Services Used | Details | Operation |
|---|---|---|
| Catalog Antibody | … 15% fetal bovine serum (Hyclone), 10ng/ml leukaemia inhibitory factor (LIF) (Z03077, Gen- Script), 1% penicillin … Primers are synthesized by Sangon Biotech and included in Table 1 … with specific antibody and immunoprecipitated on protein G magnetic beads (GenScript, the USA … | Get A Quote |
The facilitates chromatin transcription (FACT) complex is a histone H2A/H2B chaperone, which represses endogenous retroviruses (ERVs) and transcription of ERV-chimeric transcripts. It binds to both transcription start site and gene body region. Here, we investigated the downstream targets of FACT complex to identify the potential regulators of MERVL, which is a key 2-cell marker gene. H3K36me2 profile was positively correlated with that of FACT component Ssrp1. Among H3K36me2 deposition enzymes, was downregulated after the loss of . Furthermore, we demonstrated that Nsd2 repressed the expression of ERVs without affecting the expression of pluripotency genes. The expression of MERVL and 2-cell genes was partial... More
