| Products/Services Used | Details | Operation |
|---|---|---|
| Peptide Tools | Intracellular cytokine staining was employed to quantify EV71 antigen-specific T cells in immunized mice. Briefly, a total of 1,000,000 mouse splenocytes were co-stimulated with an overlapping VP1 peptide pool (1.5 μg/mL for each peptide, GenScript) for 1 hour at 37 °C in the presence of 5% CO2 along with anti-mouse CD28 antibody (Biolegend) and CD49d antibody (Biolegend), both at a concentration of 1 μg/mL. | Get A Quote |
Human Enterovirus 71 (EV71) has emerged as one of the predominant causative agents of hand, foot and mouth disease (HFMD) with global impact. Despite the inactivated vaccine being licensed, other vaccine candidates based on advanced technology platforms are under development. In this report, we rationally designed and constructed two DNA-launched live attenuated vaccine candidates (pDL-EV71) under the control of specific promoters. In vitro and in vivo transfection with pDL-EV71 driven by the CMV promoter successfully yielded fully infectious EV71. More importantly, the administration of pDL-EV71 did not cause clinical symptoms following intracranial or intramuscular inoculation in neonatal and IFNα/βR-/- mic... More
