| Source |
E.coli that carries the cloned bsaIR gene from Geobacillus stearothermophilus. |
| Recognizes sites |
5'---
G G T C T C (N)1▼ ---3' 3'--- C C A G A G (N)5▲ ---5' |
| Concentration | 10 U/µL |
| Key Features |
· No star activity under suggested usage condition; · Type IIS restriction enzymes target asymmetric DNA sequences and cleave outside their recognition sites; · Accelerated digestion – Delivers precise digestion in 15 minutes; · Animal free product. |
| Components |
|
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| Unit Definition |
One unit is defined as the amount of BsaI
required to digest 1 µg of pUC57-GS DNA1 in 1 hour at 37°C in 50 µL
of recommended reaction buffer. 1 pUC57-GS DNA is a plasmid that Genscript designed for restriction enzymes’ activity assay. |
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| 反应缓冲液 |
*The reaction buffer is intended for In vitro digestion assays of this product only, and not intended for other
applications. |
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| Storage & Stability |
Store at -20°C for up to 12 months from the date
of manufacture. Avoid repeated freeze-thaw cycles. |
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Research
use only.
