| Source |
E.coli that carries the
cloned esp3IR gene from Bacillus stearothermophilu. |
| Recognizes sites |
5'---
C G T C T C (N)1▼ ---3' 3'--- G C A G A G (N)5▲ ---5' |
| Concentration | 10U/µL |
| Key Features |
·Accelerated digestion – Delivers precise digestion in 15 minutes; ·Type IIS restriction enzymes target asymmetric DNA sequences and cleave outside their recognition sites; ·Optimal cleavage temperature is 55°C, with 70% activity preserved at 37°C; ·Animal free product. |
| Components |
|
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| Unit Definition |
One unit is
defined as the amount of BsmBI required to digest 1 µg of pUC-57-GS1
in 1 hour at 55°C in 50 µL of recommended
reaction buffer. 1 pUC57-GS DNA is a plasmid that Genscript designed for restriction enzymes’ activity assay. |
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| 反应缓冲液 | * The reaction buffer is intended for In vitro digestion assays of this product only, and not intended for other applications. | ||||||||||||
| Storage & Stability |
Store at -20 °C for up to 12 months from the
date of manufacture. Avoid repeated freeze-thaw cycles. |
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Research
use only
