Lane 1: 2 μg of TEV Protease, His, non-reducing (NR)
Lane 2: 2 μg of TEV Protease, His, reducing (R)
> 95% as analyzed by SDS-PAGE
TEV Protease, His
Z03030 | |
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¥480 | |
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联系我们 |
Lane 1: 2 μg of TEV Protease, His, non-reducing (NR)
Lane 2: 2 μg of TEV Protease, His, reducing (R)
> 95% as analyzed by SDS-PAGE
Z03030 | |
|
|
¥480 | |
|
|
|
|
联系我们 |
Tobacco Etch Virus Protease is a highly site-specific cysteine protease that is found in the tags from fusion proteins. The optimal temperature for cleavage is 30°C. It is recommended that the cleavage for each fusion protein be optimized by varying the amount of recombinant viral TEV protease, reaction time, or incubation temperature. It can be removed by Ni2+ affinity resin. |
rTEV, TEV, P1 protease
For research use only
Source | E. coli |
Purity | > 95% by SDS-PAGE analyses. |
Biological Activity | 10 IU/μl. Unit Definition: One unit of TEV protease cleaves > 85% of 3 μg of control substrate in 1 hour at pH 8.0 at 30°C. |
Formulation | Sterile liquid solution contains 50mM Tris, 5mM DTT, 50% glycerol, pH7.5. |
Measured Molecular Weight | 28.4 kDa, observed by reducing SDS-PAGE. |
Storage & Stability | Recombinant Tobacco Etch Virus Protease (rTEV) remains stable up to 1 year at -20°C from date of receipt. Please avoid freeze-thaw cycles. |