Lanes:
M.Protein Marker
1. Whole cell lysate
2. HA tagged fusion protein was precipitated by Anti-HA IP Resin
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Principle Material Supplied Genscript Anti-HA IP Resin is an agarose beads coupled to THE™ HA Tag Antibody, mAb, Mouse (Genscript, A01244). The antibody recognizes C-terminal, N-terminal, and internal HA tagged-fusion proteins. It is supplied as a suspension in 50% glycerol containing 10 mM sodium phosphate, 150 mM sodium chloride, pH 7.4, 0.02 % (w/v) sodium azide (PBS/A). Storage and Stability Store the resin: Anti-HA IP Resin is supplied at -20°C.Do not store the gel at freezing temperatures in the absence of glycerol. Store the Column: Wash the column three times with 5 ml of TBS/A (TBS containing 0.02 % sodium azide) then add another 5 ml of TBS/A and store at 2-8°C without draining. Additional Buffers Needed Equilibration buffer: 50 mM Tris, 150 mM NaCl, pH 7.4. 1×SDS Sample Buffer: 62.5 mM Tris-HCl (pH 6.8 at 25°C), 2% w/v SDS, 10% glycerol, 50 mM DTT, 0.01% w/v bromophenol blue. |
| Product Information |
Characteristics of Anti-HA IP Resin
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Lanes:
M.Protein Marker
1. Whole cell lysate
2. HA tagged fusion protein was precipitated by Anti-HA IP Resin
For
laboratory research use only. Direct human use, including taking orally and
injection and clinical use are forbidden.
