Figure 1: Neurotensin-induced concentration-dependent stimulation of intracellular calcium mobilization in CHO-K1/NTS1 cells. The cells were loaded with Calcium-4 prior to being stimulated with the agonist, neurotensin. The intracellular calcium change was measured by FLIPR^TETRA. The relative fluorescent units (RFU) were normalized and plotted against the log of the cumulative doses of neurotensin (Mean ± SEM, n = 2). The EC₅₀ of neurotensin on this cell was 7.4 nM.
Note:
1. EC₅₀ value is calculated with four parameter logistic equation:
Y=Bottom + (Top-Bottom)/(1+10^((LogEC₅₀-X)*HillSlope))
X is the logarithm of concentration.
Y is the response and starts at Bottom and goes to Top with a sigmoid shape.
2. Signal to Background Ratio (S/B) = Top/Bottom

CHO-K1/NTS1 Stable Cell Line

Neurotensin receptor 1 (NTS1) is a member of G-protein coupled receptor family A and is a receptor for Neurotensin (NT). Neurotensin (NT) exerts its intracellular effect by interacting with 3 different receptors. Two of these receptors (NTR1 and NTR2) belong to the G protein-coupled receptor family, whereas the third one (NTR3) is a type I receptor with a single transmembrane domain. The NTS1 is expressed in CNS such as cerebral cortex, basal ganglia, limbic areas, vestibular system, and esophageal smooth muscle.

产品编号	M00194
规格	2 vials 大单询价
数量
价格	询价


联系我们

Synonyms

Human Recombinant NTS1 Neurotensin Receptor Stable Cell Line

产品概述

Applications

Functional assay for NTS1 receptor

产品简介

Storage	Liquid nitrogen immediately upon delivery
Species	Human

培养条件

Freeze Medium	45% culture medium, 45% FBS (Cat. #10099-141, Gibco), 10% DMSO (Cat. #D2650, Sigma)
Culture Medium	Ham’s F-12K (Kaighn’s), 10% FBS, 400 μg/ml G418 (Cat. #10131-035, Gibco)

图例

Figure 1: Neurotensin-induced concentration-dependent stimulation of intracellular calcium mobilization in CHO-K1/NTS1 cells. The cells were loaded with Calcium-4 prior to being stimulated with the agonist, neurotensin. The intracellular calcium change was measured by FLIPR^TETRA. The relative fluorescent units (RFU) were normalized and plotted against the log of the cumulative doses of neurotensin (Mean ± SEM, n = 2). The EC₅₀ of neurotensin on this cell was 7.4 nM.
Note:
1. EC₅₀ value is calculated with four parameter logistic equation:
Y=Bottom + (Top-Bottom)/(1+10^((LogEC₅₀-X)*HillSlope))
X is the logarithm of concentration.
Y is the response and starts at Bottom and goes to Top with a sigmoid shape.
2. Signal to Background Ratio (S/B) = Top/Bottom

CHO-K1/NTS1 Stable Cell Line

资源中心

技术支持

关于我们