Figure 1. Oxotremorine M-induced concentration-dependent stimulation of intracellular calcium mobilization in CHO-K1/M4/Gα15 cells. The cells were loaded with Calcium-4 prior to be stimulated with an M4 receptor agonist, oxotremorine M. The intracellular calcium change was measured by FLIPR^TETRA. The relative fluorescent units (RFU) were plotted against the log of the cumulative doses (5-fold dilution) of oxotremorine (Mean ± SD, n = 2). The EC₅₀ of oxotremorine M on this cell was 88.7 nM.
Notes:
1. EC₅₀ value is calculated with four parameter logistic equation:
Y=Bottom + (Top-Bottom)/(1+10^((LogEC₅₀-X)*HillSlope))
X is the logarithm of concentration. Y is the response
Y is RFU and starts at Bottom and goes to Top with a sigmoid shape.
2. Signal to background Ratio (S/B) = Top/Bottom

CHO-K1/M4/Gα15 Stable Cell Line

Muscarinic acetylcholine receptors belong to a superfamily of seven-TM-domain receptors that interact with G-proteins to initiate intracellular responses. Five muscarinic receptor subtypes have been identified and named from M1 to M5. The M4 muscarinic receptor couples to Gi/o to inhibit cAMP production. GenScript co-transfected human M4 with Gα15 in the CHO-K1 which supports high levels of recombinant M4 expression on the cell surface and contains high levels of Gα15 to couple the receptor to the calcium signaling pathway.

产品编号	M00238
规格	2 vials 大单询价
数量
价格	询价


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Synonyms

M4 receptor, m4, HM3, acetylcholine receptor, muscarinic 4, cholinergic receptor, muscarin 4, muscarinic acetylcholine receptor M4, Chrm-4, muscarinic acetylcholine receptor 4

产品概述

Applications

Functional assays for M4 receptor

产品简介

Storage	Liquid nitrogen immediately upon delivery
Species	Human

培养条件

Freeze Medium	45% culture medium, 45% FBS (Cat. #10099-141, Gibco), 10% DMSO (Cat. #D2650, Sigma)
Culture Medium	Ham’s F-12K (Kaighn’s), 10% FBS, 200 μg/ml Zeocin (Cat. #R250-01, Life Technologies), 100 μg/ml Hygromycin B (Cat. #10687010, Invitrogen)

图例

Figure 1. Oxotremorine M-induced concentration-dependent stimulation of intracellular calcium mobilization in CHO-K1/M4/Gα15 cells. The cells were loaded with Calcium-4 prior to be stimulated with an M4 receptor agonist, oxotremorine M. The intracellular calcium change was measured by FLIPR^TETRA. The relative fluorescent units (RFU) were plotted against the log of the cumulative doses (5-fold dilution) of oxotremorine (Mean ± SD, n = 2). The EC₅₀ of oxotremorine M on this cell was 88.7 nM.
Notes:
1. EC₅₀ value is calculated with four parameter logistic equation:
Y=Bottom + (Top-Bottom)/(1+10^((LogEC₅₀-X)*HillSlope))
X is the logarithm of concentration. Y is the response
Y is RFU and starts at Bottom and goes to Top with a sigmoid shape.
2. Signal to background Ratio (S/B) = Top/Bottom

CHO-K1/M4/Gα15 Stable Cell Line

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