目录产品 » 稳定细胞系 » Human Recombinant Platelet Activating Factor Receptor Stable Cell Line
CHO-K1/PTAFR Stable Cell Line

Figure 1. PAF-induced concentration-dependent stimulation of intracellular calcium mobilization in CHO-K1/PTAFR cells. The cells were loaded with Calcium-4 prior to being stimulated with agonist PAF. The intracellular calcium change was measured by FLIPR. The relative fluorescent units (RFU) were normalized and plotted against the log of the cumulative doses (5-fold dilution) of PAF (Mean ± SD, n = 2). The EC50 of PAF on the cells was 0.13 μM.
Notes:
1. EC50 value is calculated with four parameter logistic equation:
Y=Bottom + (Top-Bottom)/ (1+10^ ((LogEC50-X)*HillSlope))
X is the logarithm of concentration.
Y is RFU and starts at Bottom and goes to Top with a sigmoid shape.

CHO-K1/PTAFR Stable Cell Line

The platelet-activating factor (PAF) receptor (PTAFR) is a G protein coupled receptor that signals through multiple pathways and mediates several cellular responses including cell motility, smooth muscle contraction, and releases of cytokine and leukotriene (Stafforini et al., 2003). In humans, various diseases have been associated with PAF, such as allergic asthma, endotoxic shock, atherosclerosis and psoriasis.
M00262
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Synonyms

Human Recombinant Platelet Activating Factor Receptor Stable Cell Line

Applications Functional assays for PAF receptor

Storage Liquid nitrogen immediately upon delivery
Species Human

Freeze Medium 45% culture medium, 45% FBS (Cat. #10099-141, Gibco), 10% DMSO (Cat. #D2650, Sigma)
Culture Medium Ham’s F-12K (Kaighn’s), 10% FBS, 200 μg/ml Zeocin (Cat. #R250-01, Life Technologies)

  • CHO-K1/PTAFR Stable Cell Line
  • CHO-K1/PTAFR Stable Cell Line

    Figure 1. PAF-induced concentration-dependent stimulation of intracellular calcium mobilization in CHO-K1/PTAFR cells. The cells were loaded with Calcium-4 prior to being stimulated with agonist PAF. The intracellular calcium change was measured by FLIPR. The relative fluorescent units (RFU) were normalized and plotted against the log of the cumulative doses (5-fold dilution) of PAF (Mean ± SD, n = 2). The EC50 of PAF on the cells was 0.13 μM.
    Notes:
    1. EC50 value is calculated with four parameter logistic equation:
    Y=Bottom + (Top-Bottom)/ (1+10^ ((LogEC50-X)*HillSlope))
    X is the logarithm of concentration.
    Y is RFU and starts at Bottom and goes to Top with a sigmoid shape.




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