Figure 1. Cortistatin 14-induced concentration-dependent stimulation of intracellular calcium mobilization in CHO-K1/MRGPRX2 cells. The cells were loaded with Calcium-4 prior to being stimulated with agonist Cortistatin 14. The intracellular calcium change was measured by FLIPR. The relative fluorescent units (RFU) were normalized and plotted against the log of the cumulative doses (5-fold dilution) of Cortistatin 14 (Mean ± SD, n = 2). The EC₅₀ of Cortistatin 14 on this cell was 0.64 µM.
Notes:
1. EC₅₀ value is calculated with four parameter logistic equation:
Y=Bottom + (Top-Bottom)/(1+10^((LogEC₅₀-X)*HillSlope))
X is the logarithm of concentration. Y is the response
Y is RFU and starts at Bottom and goes to Top with a sigmoid shape.

CHO-K1/MRGPRX2 Stable Cell Line

Recombinant CHO-K1 cells stably overexpress mas-related G-protein coupled receptor member X2 (MRGPRX2). MRGPRX2 couples to both Gi and Gq alpha subunits in mast cells naturally, so that the PLC signaling can be triggered within the cells by agonist binding. This cell line is recommended for agonist screening and functional validations with calcium flux assay.

产品编号	M00425
规格	2 vials 大单询价
数量
价格	询价


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产品概述

Product Description	Recombinant CHO-K1 cells stably overexpress mas-related G-protein coupled receptor member X2 (MRGPRX2). MRGPRX2 couples to both Gi and Gq alpha subunits in mast cells naturally, so that the PLC signaling can be triggered within the cells by agonist binding. This cell line is recommended for agonist screening and functional validations with calcium flux assay.
Applications	Agonist screening and functional validations of MRGPRX2.

产品简介

Expressed Gene	Codon Optimized from NM_054030; no expressed tags
Target Protein	NP_473371
Host Cell	CHO-K1
Quantity	2 vials of frozen cells (>1X10⁶ per vial in 1 ml)
Storage	Store cells in liquid nitrogen immediately upon receipt. Thaw and recover cells within one year from the date received.
Mycoplasma Status	Negative. The mycoplasma test was performed with MycoAlert™ PLUS Mycoplasma Detection Kit (Cat. No. LT07-318, Lonza).
Stability	Stable through more than 15 passages with no significant changes in assay performance or expression profile.

培养条件

Culture Properties	Adherent
Freeze Medium	45% culture medium, 45% FBS (Cat. No. 10099-141, Gibco), 10% DMSO (Cat. No. D2650, Sigma)
Complete Growth Medium	Ham’s F-12K (Kaighn’s) Medium (Cat. No. 21127-022, Gibco), 10% FBS (Cat. No. 10099-141, Gibco)
Culture Medium	Ham’s F-12K (Kaighn’s) Medium (Cat. No. 21127-022, Gibco), 10% FBS (Cat. No. 10099-141, Gibco), 200 μg/ml Zeocin (Cat. No. R250-01, Life Technologies)

技术背景

Gene Synonyms

MRGPRX2; MRGX2

Background

MRGPRX2 (also known as MRGX2) is a Gq and Gi-coupled GPCR that binds to several peptides, including the neuropeptide cortistatin-14 and the proadrenomedullin N-terminal peptide (PAMP). It is probably involved in the function of nociceptive neurons and regulation of nociceptor function and/or development, including the sensation or modulation of pain. Dorsal root ganglia and adrenal chromaffin cells express MRGPRX2, and binding of these ligands to MRGPRX2 is thought to play a role in nociception and catecholamine release from the adrenal glands.

图例

CHO-K1/MRGPRX2 Stable Cell Line

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