| Product Description |
GenScript Protein L Resin is an affinity chromatography medium designed for easy, one-step purification of classes, subclasses and fragments of immunoglobulins from biological fluids and from cell culture media. The highly purified protein L ligand is coupled to 4% agarose. The coupling is optimized to give high binding capacity for immunoglobulins. The static binding capacity of Protein L Resin is greater than 15 mg Human IgG/ml settled resin. The dynamic binding capacity will vary depending on several factors such as target antibody, flow rate etc. Table 1 lists the characteristics of Protein L Resin.
Protein L, first isolated from the surface of bacterial species Peptostreptoccus magnus, binds immunoglobulin through κ light chain interaction. Protein L binds a wider range of Ig classes and subclasses than other antibody-binding proteins such as protein A or protein G. It can bind to all classes of Ig (i.e., IgG, IgM, IgA, IgE, and IgD) and also the single chain variable fragments (Scfv) and Fab fragments. The binding properties of Protein L to antibodies and antibody fragments of different species and subtypes are shown in Table 2.
|
| Matrix Spherical |
4% agarose |
| Average particle size |
90 μm (45-165 μm) |
| Ligand |
highly purified protein L |
| Binding Capacity |
≥ 15 mg Human IgG/ml settled resin |
| Storage Solution |
1 × PBS containing 20% ethanol |
| Storage & Stability |
2-8 °C; DO NOT FREEZE |
For research and manufacturing use. Direct human use, including taking orally and injection are forbidden.
