Regeneration performance of MonoRab™ Anti-VHH Affinity Magnetic Beads

The target VHH was captured by GenScript’s MonoRab™ Anti-VHH Affinity Magnetic Beads (Cat. No. L01034). The 100 μL settled beads were washed twice with 1 mL TBS and then eluted with 1 mL acidic eluent (0.1 M glycine HCl, pH 2.5). Subsequently, the beads were regenerated by 1 mL regeneration buffer for reuse. The coefficient of variation (CV) of the amount of eluted target protein was less than 20% for a total of 10 replicate purifications indicating that GenScript’s MonoRab™ Anti-VHH Affinity Magnetic Beads is capable of multiple repeated use with relatively high consistency when eluted by acidic eluent.

Purification of humanized VHH from E.coli whole cell lysate

The humanized VHH (M.W.=15 kDa) from E.coli whole cell lysate was captured by GenScript’s MonoRab™ Anti-VHH Affinity Magnetic Beads (Cat. No. L01034). The captured humanized VHH was subsequently eluted using an acidic eluent (0.1 M glycine HCl, pH 2.5) and subjected to SDS-PAGE analysis. The results showed that GenScript’s MonoRab™ Anti-VHH Affinity Magnetic Beads are suitable for high-specificity purification of humanized VHH from E.coli whole cell lysate
Lane 1: Broad Multi Color Pre-Stained Protein Standard (GenScript, M00624)
Lane 2: E.coli whole cell lysate containing humanized VHH
Lane 3: Flow-through of E.coli whole cell lysate containing humanized VHH
Lane 4: Eluate of target humanized VHH from E.coli whole cell lysate with L01034

Purification of camelid VHH from various lysates

The camelid VHH (M.W.=15 kDa ) from E.coli whole cell lysate, yeast whole cell lysate or mammalian cell culture supernatant was captured by GenScript’s MonoRab™ Anti-VHH Affinity Magnetic Beads (Cat. No. L01034). The captured camelid VHH was subsequently eluted using an acidic eluent (0.1 M glycine HCl, pH 2.5) and subjected to SDS-PAGE analysis. The results showed that GenScript’s MonoRab™ Anti-VHH Affinity Magnetic Beads are suitable for high-specificity purification of camelid VHH from widely used protein expression systems.
Lane 1: Broad Multi Color Pre-Stained Protein Standard (GenScript, M00624)
E.coli：
Lane 2: E.coli whole cell lysate containing camelid VHH
Lane 3: Flow-through of E.coli whole cell lysate containing camelid VHH
Lane 4: Eluate of target camelid VHH from E.coli whole cell lysate with L01034
Yeast：
Lane 5: Yeast whole cell lysate containing camelid VHH
Lane 6: Flow-through of yeast whole cell lysate containing camelid VHH
Lane 7: Eluate of target camelid VHH from yeast whole cell lysate with L01034
Mammalian：
Lane 8: Mammalian cell culture supernatant containing camelid VHH
Lane 9: Flow-through of mammalian cell culture supernatant containing camelid VHH
Lane 10: Eluate of target camelid VHH from mammalian cell culture supernatant with L01034