| Species |
Human |
| Protein Construction |
IL-15 (Asn49-Ser162)
Accession # P40933 |
|
| Purity |
> 95% as analyzed by SDS-PAGE
> 95% as analyzed by HPLC |
| Endotoxin Level |
< 1 EU/μg of protein by LAL method |
| Biological Activity |
The ED50 as determined by the dose-dependant stimulation of the proliferation of CTLL-2 was found to be < 0.5 ng/ml, corresponding to a specific activity of > 2.0 × 106 IU/mg. |
| Expression System |
E. coli |
| Theoretical Molecular Weight |
12.5 kDa |
| N-terminal Sequence Analysis |
The sequence of the first five N-terminal amino acids was determined and found to be Asn-Trp-Val-Asn-Val. |
| Formulation |
Lyophilized after extensive dialysis against 20 mM PB, pH 7.5, 150 mM NaCl buffer. |
| Reconstitution |
It is recommended that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute the lyophilized powder in sterile 18 MΩ-cm H₂O up to 100 µg/ml. |
| Storage & Stability |
Upon receiving, this product remains stable for up to 6 months at -70°C or -20°C. Upon reconstitution, the product should be stable for up to 1 week at 4°C or up to 3 months at -20°C. Avoid repeated freeze-thaw cycles. |
The ED50 as determined by the dose-dependent stimulation of the proliferation of CTLL-2 was found to be < 0.5 ng/ml, corresponding to a specific activity of > 2.0 × 106 IU/mg.
Lane 1: 2 μg of IL-15, Human, reducing (R)
Lane 2: 2 μg of IL-15, Human, non-reducing (NR)
> 95% as analyzed by SDS-PAGE
| Target Background |
Interleukin-15 (IL-15) is a cytokine with structural similarity to IL-2. Like IL-2, IL-15 binds to and signals through a complex composed of IL-2/IL-15 receptor beta chain (CD122) and the common gamma chain (gamma-C, CD132). IL-15 is secreted by mononuclear phagocytes (among other cells) following infection by virus. This cytokine induces cell proliferation of natural killer cells, which are cells of the innate immune system whose principal role is to kill virally infected cells. IL-15 can stimulate the proliferation of T-lymphocytes. Stimulation by IL-15 occurs following its interaction with IL-15Rα. This interaction may enhance IL-15’s interaction with IL15Rβγc. |
| Synonyms |
IL15; IL-T; interleukin 15 |
For laboratory research use only. Direct human use, including taking orally and injection and clinical use are forbidden.
