| Species |
Human |
| Protein Construction |
PTH (Ser32-Gln115)
Accession # P01270 |
|
| Purity |
> 95% as analyzed by SDS-PAGE |
| Endotoxin Level |
< 1 EU/μg of protein by LAL method |
| Expression System |
E. coli |
| Theoretical Molecular Weight |
~9.4 kDa |
| Apparent Molecular Weight |
~14 kDa, on SDS-PAGE under non-reducing conditions. |
| Formulation |
Lyophilized from a 0.2 μm filtered solution of 10mM HAc-NaAc, 150mM NaCl, 5% Mannitol, pH 4.0. |
| Reconstitution |
It is recommended that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute the lyophilized powder in distilled water up to 100 μg/ml. |
| Storage & Stability |
Upon receiving, this product remains stable for up to 6 months at -70°C or -20°C. Upon reconstitution, the product should be stable for up to 1 week at 4°C or up to 3 months at -20°C. Avoid repeated freeze-thaw cycles. |
Lane 1: 2 μg of PTH (1-84aa), Human, non-reducing (NR)
Lane 2: 2 μg of PTH (1-84aa), Human, reducing (R)
> 95% as analyzed by SDS-PAGE
| Target Background |
Parathyroid Hormone (PTH) is the most important endocrine regulator of calcium and phosphorus con- centration in extracellular fluid. Parathyroid Hormone (PTH) is secreted from cells of the parathyroid glands and finds Parathyroid Hormone’s major target cells in bone and kidney. Like most other protein hormones, Parathyroid Hormone (PTH) is synthesized as a preprohormone. After intracellular processing, the mature hormone is packaged within the Golgi into secretory vesicles, the secreted into blood by exocytosis. Parathyroid Hormone (PTH) is secreted as a linear protein of 84 amino acids. Recombinant Human Parathyroid Hormone (PTH) produced in E. coli is a single, non-glycosylated, polypeptide chain containing 84 amino acids. |
| Synonyms |
Parathyroid Hormone |
For laboratory research use only. Direct human use, including taking orally and injection and clinical use are forbidden.
