| Species |
Human |
| Protein Construction |
CD40L (Met113-Leu261)
Accession # P29965 |
|
| Purity |
> 95% as analyzed by SDS-PAGE
> 95% as analyzed by RP-HPLC
> 95% as analyzed by Anion-exchange FPLC |
| Endotoxin Level |
< 1 EU/μg of protein by LAL method |
| Biological Activity |
Recombinant human CD40 Ligand (CD40L/CD154/TRAP) is fully biologically active when compared to standard. The ED50 as determined by the dose-dependant stimulation of IL-12 & IL-8 induction by PMB (Peripheral Mononuclear) cells was found to be 5.0-10.0 ng/ml, corresponding to a Specific Activity of 1.0 x 105 IU/mg. |
| Expression System |
E. coli |
| Theoretical Molecular Weight |
16.3 kDa |
| N-terminal Sequence Analysis |
The sequence of the first five N-terminal amino acids was determined and found to be Met-Gln-Lys-Gly-Asp. |
| Formulation |
Lyophilized after extensive dialysis against 50 mM Tris-HCl, pH 8.0, 250 mM NaCl buffer. |
| Reconstitution |
It is recommended that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute the lyophilized powder in sterile 18 MΩ-cm H₂O up to 100 µg/ml. |
| Storage & Stability |
Upon receiving, this product remains stable for up to 6 months at -70°C or -20°C. Upon reconstitution, the product should be stable for up to 1 week at 4°C or up to 3 months at -20°C. Avoid repeated freeze-thaw cycles. |
| Target Background |
CD40 Ligand (CD40L/CD154/TRAP) is a membrane glycoprotein and differentiation antigen expressed on the surface of T-cells. The CD40 ligand stimulates B-cell proliferation and secretion of all immunoglobulin isotypes in the presence of cytokines. It also costimulates proliferation of activated T-cell and this is accompanied by the production of IFN-γ, TNF-α, and IL2. CD40 ligand has been shown to induce cytokine production and tumoricidal activity in peripheral blood monocytes. |
| Synonyms |
CD40 ligand; CD40-L; T-cell antigen Gp39; TNF-related activation protein; Tumor necrosis factor ligand superfamily member 5; CD_antigen: CD154; CD40LG; TNFSF5 |
For laboratory research use only. Direct human use, including taking orally and injection and clinical use are forbidden.
