Pulldown assay is a conventional method to determine protein-protein interactions in vitro.
Expressing a protein of interest with two different tags allows testing whether both versions can be
captured via one of the two tags as homooligomeric complex. This protocol is based on streptavidin
bead capture of a biotinylated protein and co-associated Flag-tagged protein using Streptavidin
MagBeads.
Pulldown assay is a conventional method to determine protein-protein interactions in vitro.
Expressing a protein of interest with two different tags allows testing whether both versions can be
captured via one of the two tags as homooligomeric complex. This protocol is based on streptavidin
bead capture of a biotinylated protein and co-associated Flag-tagged protein using Streptavidin
MagBeads.
