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Improved fatty aldehyde and wax ester production by overexpression of fatty acyl-CoA reductases.

Microb. Cell Fact.. 2018-02; 
LehtinenTapio,EfimovaElena,SantalaSuvi,SantalaV
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Codon Optimization … Aar (S. elongatus PCC7942_orf 1594) was codon-optimized and purchased from Genscript (USA) with appropriate restriction sites and ribosomal binding site. The three genes were separately cloned to the Biobrick cloning site of the constructed cassette … Get A Quote

摘要

Fatty aldehydes are industrially relevant compounds, which also represent a common metabolic intermediate in the microbial synthesis of various oleochemicals, including alkanes, fatty alcohols and wax esters. The key enzymes in biological fatty aldehyde production are the fatty acyl-CoA/ACP reductases (FARs) which reduce the activated acyl molecules to fatty aldehydes. Due to the disparity of FARs, identification and in vivo characterization of reductases with different properties are needed for the construction of tailored synthetic pathways for the production of various compounds.

关键词

Acinetobacter baylyi ADP1,FAR,Fatty acyl-CoA reductase,Fatty aldehyde,Wax e