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Codon Optimization | … Materials and methods. Preparation of ZIKV NS2B-NS3 pro. ZIKV NS2B-NS3 pro gene was synthesized after codon optimization (Genscript, Piscataway, NJ, USA) for expression in E. coli based on amino acid sequence of NS2B-NS3 pro (GenBank Accession No … | Get A Quote |