| Products/Services Used | Details | Operation |
|---|---|---|
| Codon Optimization | … For the mvk mcl , mvk mpd and mvk mma genes, codon optimization (the substitution of codons that are rare in E. coli with synonymous codons that are more frequently used) was performed using OptimumGene provided by GenScript … | Get A Quote |
The inhibition of mevalonate kinase (MVK) by downstream metabolites is an important mechanism in the regulation of isoprenoid production in a broad range of organisms. The first feedback-resistant MVK was previously discovered in the methanogenic archaeon Methanosarcinamazei. Here， we report the cloning， expression， purification， kinetic characterization and inhibition analysis of MVKs from two other methanogens， Methanosaetaconcilii and Methanocellapaludicola. Similar to the M. mazei MVK， these enzymes were not inhibited by diphosphomevalonate (DPM)， dimethylallyl diphosphate (DMAPP)， isopentenyldiphosphate (IPP)， geranylpyrophosphate (GPP) or farnesylpyrophosphate (FPP). However， they exhi... More
