Viral protein expression in Escherichia coli (E. coli) is a powerful tool for structural/functional studies as well as for vaccine and diagnostics development. However， numerous factors such as codon bias， mRNA secondary structure and nucleotides distribution， have been indentified to hamper this heterologous expression.
Viral protein expression in Escherichia coli (E. coli) is a powerful tool for structural/functional studies as well as for vaccine and diagnostics development. However， numerous factors such as codon bias， mRNA secondary structure and nucleotides distribution， have been indentified to hamper this heterologous expression.
