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Combined engineering of disaccharide transport and phosphorolysis for enhanced ATP yield from sucrose fermentation in Saccharomyces cerevisiae.

Metab. Eng.. 2018; 
MarquesWesley Leoricy,MansRobert,HendersonRyan K,MarellaEko Roy,HorstJolanda Ter,HulsterErik de,PoolmanBert,DaranJean-Marc,PronkJack T,GombertAndreas K,van MarisAntonius
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Codon Optimization PvSUF1 (NCBI accession number DQ221700.1) was codon optimized for expression in S. cerevisiae with OptimumGene™ (GenScript, Supplementary material), purchased from GenScript 14 USA Inc. (Piscataway, NJ) and delivered within pUC57-PvSUF1 Get A Quote

摘要

Anaerobic industrial fermentation processes do not require aeration and intensive mixing and the accompanying cost savings are beneficial for production of chemicals and fuels. However, the free-energy conservation of fermentative pathways is often insufficient for the production and export of the desired compounds and/or for cellular growth and maintenance. To increase free-energy conservation during fermentation of the industrially relevant disaccharide sucrose by Saccharomyces cerevisiae, we first replaced the native yeast α-glucosidases by an intracellular sucrose phosphorylase from Leuconostoc mesenteroides (LmSPase). Subsequently, we replaced the native proton-coupled sucrose uptake system by a put... More

关键词

ATP,Chemostat,Facilitated diffusion,Free-energy conservation,Phosphoglucomutase,Yeast physio