至今,GenScript的服务及产品已被Cell, Nature, Science, PNAS等1300多家生物医药类杂志引用近万次,处于行业领先水平。NIH、哈佛、耶鲁、斯坦福、普林斯顿、杜克大学等约400家全球著名机构使用GenScript的基因合成、多肽服务、抗体服务和蛋白服务等成功地发表科研成果,再次证明GenScript 有能力帮助业内科学家Make research easy.

Expressing accessory proteins in cellulolytic to improve the conversion yield of recalcitrant cellulose.

Biotechnol Biofuels. 2017; 
GuoZhong-Peng,DuquesneSophie,BozonnetSophie,NicaudJean-Marc,MartyAlain,O'DonohueMichael Jo
Products/Services Used Details Operation
Codon Optimization The SWO1 gene (GenBank accession code: AJ245918.1), encoding T. reesei swollenin, was synthesized by GenScript (USA) after codon optimization based on the codon bias of Y.lipolytica (Additional file 2), and cloned into the plasmid JMP62UraTEF.Blots were sequentially treated with mouse non-position-specific His-Tag antibody 1:2500 (THE™ from Genscript, Piscataway, NJ, US) and the alkaline phosphatase-conjugated goat anti-mouse IgG. Phosphatase activity was detected by NBT/BCIP as substrate. Get A Quote

摘要

A recently constructed cellulolytic is able to grow efficiently on an industrial organosolv cellulose pulp, but shows limited ability to degrade crystalline cellulose. In this work, we have further engineered this strain, adding accessory proteins xylanase II (XYNII), lytic polysaccharide monooxygenase (LPMO), and swollenin (SWO) from in order to enhance the degradation of recalcitrant substrate.

关键词

Accessory proteins,Cellulolytic biocatalyst,Consolidated bioprocessing,Lytic polysaccharide monooxygenase,Swollenin,Xylanase,Yarrowia lipoly