Yeast Yarrowia lipolytica is a handy tool for efficient
production of heterologous proteins. In this work we
report the construction of integration vectors for
secretion of bacterial phytase by t hese yeasts. Pantoea
sp. 3.5.1 histidine acid phytase encoding gene sequence
was codon-optimized and chemically synthetized.
Optimized and native phytase gene sequences were
cloned into integrative vector pINA1296, containing
signal sequence of XPR2 gene. Vectors were multiplied
in E.coli DH5Į, isolated and linearized for successful
integration into Y.lipolytica genome by homologeous
recombination in pBR-region. Y.lipolytica strains with
integrated bacterial native and optimized phytase genes
were obtained
Yeast Yarrowia lipolytica is a handy tool for efficient
production of heterologous proteins. In this work we
report the construction of integration vectors for
secretion of bacterial phytase by t hese yeasts. Pantoea
sp. 3.5.1 histidine acid phytase encoding gene sequence
was codon-optimized and chemically synthetized.
Optimized and native phytase gene sequences were
cloned into integrative vector pINA1296, containing
signal sequence of XPR2 gene. Vectors were multiplied
in E.coli DH5Į, isolated and linearized for successful
integration into Y.lipolytica genome by homologeous
recombination in pBR-region. Y.lipolytica strains with
integrated bacterial native and optimized phytase genes
were obtained
