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Codon Optimization | … for V H H R419 [11] was codon optimized for E. coli expression and produced as a double stranded GenPart DNA fragment (Genscript Inc, NJ) … Fermentation was carried out overnight (16 h, 30 °C, 225 rev min −1 ). V H H R419 was extracted from the periplasm using an osmic … | Get A Quote |