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| Codon Optimization | In addition, another two control genes (egfp-GenScript and mApple-GenScript) were designed, which mainly used the high-frequency-usage codons of E. coli designed by the GenScript software. The sequences of those genes are shown in Figs S8 and S9. The four genes (egfp-codon, mApple-codon, egfp-GenScript and mApple-GenScript) were expressed in the same E. coli expression system. The fluorescence intensity of eGFP and mApple expressed from eGFP-codon and mApple-codon was about 2.3- or 1.7- folds greater than that from eGFP-GenScript and mApple-GenScript (Fig. 5). In addition, as shown in SDS-PAGE (Fig. S10), the amount of the expressed proteins eGFP and mApple from eGFP-codon and mApple-codon was also higher than that from eGFP-GenScript and mApple-GenScript. The reporter genes (egfp-codon, mApple-codon, egfp-GenScript and mApple-GenScript) were cloned into a pET30a(+) expression vector and overexpressed in E. coli strain BL21(DE3) pLys. | Get A Quote |
Of the 20 common amino acids， 18 are encoded by multiple synonymous codons. These synonymous codons are not redundant; in fact， all of codons contribute substantially to protein expression， structure and function. In this study， the codon usage pattern of genes in the E. coli was learned from the sequenced genomes of E. coli. A machine learning based method， Presyncodon was proposed to predict synonymous codon selection in E. coli based on the learned codon usage patterns of the residue in the context of the specific fragment. The predicting results indicate that Presycoden could be used to predict synonymous codon selection of the gene in the E. coli with the high accuracy. Two reporter genes (egfp a... More
