| Products/Services Used | Details | Operation |
|---|---|---|
| Monoclonal Antibody Services | The following primary antibodies were used in western blot analysis: monoclonal anti-PERK produced in rabbit (1:500, cell signaling, #3192), monoclonal anti-β-actin produced in mouse (1:1000, GenScript, A00702) | Get A Quote |
PERK (EIF2AK3) is an ER-resident eIF2α kinase required for memory flexibility and metabotropic glutamate receptor-dependent long-term depression， processes known to be dependent on new protein synthesis. Here we investigated PERK's role in working memory， a cognitive ability that is independent of new protein synthesis， but instead is dependent on cellular Ca2+ dynamics. We found that working memory is impaired in forebrain-specific Perk knockout and pharmacologically PERK-inhibited mice. Moreover， inhibition of PERK in wild-type mice mimics the fear extinction impairment observed in forebrain-specific Perk knockout mice. Our findings reveal a novel role of PERK in cognitive functions and suggest that ... More
