The methylotrophic yeast Pichia pastoris is a common host for the production of recombinant proteins. However， hypermannosylation hinders the use of recombinant proteins from yeast in most biopharmaceutical applications. Glyco-engineered yeast strains produce more homogeneously glycosylated proteins， but can be physiologically impaired and show tendencies for cellular agglomeration， hence are hard to cultivate. Further， comprehensive data regarding growth， physiology and recombinant protein production in the controlled environment of a bioreactor are scarce.
The methylotrophic yeast Pichia pastoris is a common host for the production of recombinant proteins. However， hypermannosylation hinders the use of recombinant proteins from yeast in most biopharmaceutical applications. Glyco-engineered yeast strains produce more homogeneously glycosylated proteins， but can be physiologically impaired and show tendencies for cellular agglomeration， hence are hard to cultivate. Further， comprehensive data regarding growth， physiology and recombinant protein production in the controlled environment of a bioreactor are scarce.
