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Proteins, Expression, Isolation and Analysis | … The FPR2 antagonist WRWWWW was from Genscript Corporation (Scotch Plains, NJ, USA). The formylated fMLF, IL8, isoluminol, latrunculinA and, FITC-labeled phalloidin, were obtained from Sigma (Sigma Chemical Co., St. Louis, MO, USA) … | Get A Quote |
Formyl peptide receptor (FPR)-desensitized neutrophils display increased production/release of superoxide (O) when activated by platelet-activating factor (PAF), a priming of the response achieved through a unique receptor crosstalk mechanism. The aim of this study was to determine the effect of an inhibitor selective for small, heterotrimeric G proteins belonging to the Gαq subclass on that receptor crosstalk. We show that signals generated by FPRs and the PAF receptor (PAFR) induce activation of the neutrophil O, producing NADPH-oxidase, and that response was sensitive to Gαq inhibition in cells activated by PAF, but no inhibition was obtained in cells activated by FPR agonists. Signaling in naive... More