Products/Services Used | Details | Operation |
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Proteins, Expression, Isolation and Analysis | Samples were resolved on precast gels (GenScript), transferred onto PVDF membranes, and immunoblotted with c-Myc (9E10) antibodies (sc-40, Santa Cruz Biotechnology) at 1:1000 dilution. | Get A Quote |
Bacteria deliver toxic effectors via type VI secretion systems (T6SSs) to dominate competitors, but the identity and function of many effectors remain unknown. Here we identify a Vibrio antibacterial T6SS effector that contains a previously undescribed, widespread DNase toxin domain that we call PoNe (Polymorphic Nuclease effector). PoNe belongs to a diverse superfamily of PD-(D/E)xK phosphodiesterases, and is associated with several toxin delivery systems including type V, type VI, and type VII. PoNe toxicity is antagonized by cognate immunity proteins (PoNi) containing DUF1911 and DUF1910 domains. In addition to PoNe, the effector contains a domain of unknown function (FIX domain) that is also fou... More