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| Codon Optimization | Codon optimization and DNA synthesis were performed by GenScript and Gen9. mAb-containing supernatants from transfected expi293 cells were clarified by centrifugation and then incubated with protein A agarose resin (GenScript) in batch format overnight, followed by washing, elution, and buffer exchange into DPBS as described (Smith et al., 2009). | Get A Quote |
Ebola virus (EBOV) remains a public health threat. We performed a longitudinal study of B cell responses to EBOV in four survivors of the 2014 West African outbreak. Infection induced lasting EBOV-specific immunoglobulin G (IgG) antibodies， but their subclass composition changed over time， with IgG1 persisting， IgG3 rapidly declining， and IgG4 appearing late. Striking changes occurred in the immunoglobulin repertoire， with massive recruitment of naive B cells that subsequently underwent hypermutation. We characterized a large panel of EBOV glycoprotein-specific monoclonal antibodies (mAbs). Only a small subset of mAbs that bound glycoprotein by ELISA recognized cell-surface glycoprotein. However， th... More
