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Viral N6-methyladenosine upregulates replication and pathogenesis of human respiratory syncytial virus

Nat Commun.. 2019; 
Xue M1, Zhao BS2, Zhang Z2, Lu M1, Harder O1, Chen P3, Lu Z2, Li A1, Ma Y1, Xu Y4, Liang X1, Zhou J5, Niewiesk S1, Peeples ME3,6, He C2,7, Li J8.
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Codon Optimization In addition, M-fold and Genscript software were used to predict that these mutations did not alter RNA secondary structure or codon usage. Get A Quote

摘要

N6-methyladenosine (m6A) is the most prevalent internal modification of mRNAs in most eukaryotes. Here we show that RNAs of human respiratory syncytial virus (RSV) are modified by m6A within discreet regions and that these modifications enhance viral replication and pathogenesis. Knockdown of m6A methyltransferases decreases RSV replication and gene expression whereas knockdown of m6A demethylases has the opposite effect. The G gene transcript contains the most m6A modifications. Recombinant RSV variants expressing G transcripts that lack particular clusters of m6A display reduced replication in A549 cells, primary well differentiated human airway epithelial cultures, and respiratory tracts of cotton rats. One ... More

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