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DENV2 Pseudoviral Particles with Unprocessed Capsid Protein Are Assembled and Infectious

Viruses. 2019; 
Rana J, Burrone OR.
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Codon Optimization … The gene fragment encoding for the structural proteins (C-PrM-E) from DENV2 NGC strain (GenBank accession number AAA42941) was obtained as a synthetic, mammalian-codon optimised gene (GenScript, Piscataway, NJ, USA) and cloned into pVAX1 expression vector … Get A Quote

摘要

Proteolytic processing of flavivirus polyprotein is a uniquely controlled process. To date, the sequential cleavage of the capsid anchor sequence at the junction of C-PrM has been considered essential for the production of flaviviruses. In this study, we used two experimental approaches to show the effect of unprocessed capsid on the production and infectivity of dengue virus 2 (DENV2) pseudoviral particles. The results showed that (1) both mature and unprocessed capsids of DENV2 were equally efficient in the viral RNA packaging and also in the assembly of infective particles; (2) DENV2 variants, in which the viral and host mediated cleavage of Ca peptide were independent, produced significantly higher levels o... More

关键词

capsid anchor; dengue virus; infectivity; sequential cleavage