Products/Services Used | Details | Operation |
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Proteins, Expression, Isolation and Analysis | … Other purchase details are as follows: Lipofectamine reagent (Invitrogen), PVDF membrane (GE Healthcare/Millipore), Rapamycin (Calbiochem, USA), and Mnk1 inhibitor (Merck USA), Protein G-Agarose beads (Genscript), Glutathione agarose beads (Thermo Scientific … | Get A Quote |
Cellular signals that influence Cap-dependent translation have assumed significant relevance in the backdrop of their enforced dysregulation during oncogenesis. Eukaryotic initiation factor 4E(eIF4E), the mRNA cap-binding protein, has emerged as a key player to facilitate tumor progression through upregulated cap-dependent translation synchronized with enhanced cell division. We provide evidence that eIF4E phosphorylation is regulated by mTORC1 by virtue of its interaction with Raptor through a novel TPTPNPP motif and consequent phosphorylation invitro and in vivo in a Rapamycin-sensitive manner. While we show that phosphorylation pattern of eIF4E responds faithfully to Rapamycin inhibition, the prolonged expos... More