| Products/Services Used | Details | Operation |
|---|---|---|
| Proteins, Expression, Isolation and Analysis | The supernatant was loaded on a Nickel column (Genscript, Nanjing, China), washed with running buffer containing 50 mM Tris-HCl, 300 mM NaCl and 5% glycerol (pH 8.... Lane M, Smar t Broad-Range protein standard (Genscript, Nanjing, China); lane 1, the supernatant of un-induced cells; lane 2, the precipitant of un-induced cells; lane 3, the supernatant of the cells induced at 15˚C; lane 4, the precipitant of the cells induced at 15˚C; lane 5, the supernatant of the cells induced at 25˚C; lane 6, the precipitant of the cells induced at 25˚C; lane 7, the supernatant of the cells induced at 37˚C; lane 8, the precipitant of the cells induced at 37˚C. | Get A Quote |
Per a 9 is a major allergen of the American cockroach (CR), which has been recognized as an important cause of imunoglobulin E-mediated type I hypersensitivity worldwide. However, it is not neasy to obtain a substantial quantity of this allergen for use in functional studies. In the present study, the Per a 9 gene was cloned and expressed in Escherichia coli (E. coli) systems. It was found that 13/16 (81.3%) of the sera from patients with allergies caused by the American CR reacted to Per a 9, as assessed by enzyme-linked immunosorbent assay, confirming that Per a 9 is a major allergen of CR. The induction of the expression of CD63 and CCR3 in passively sensitized basophils (from sera of patients... More
