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| Codon Optimization | … AAC94993.1) was inserted. Moreover, a 6-His tag was added at the C-terminal site of the NG34/CS17 sequence. The whole cDNA was chemically synthesized (GenScript, Piscataway, NJ, USA) (Fig. 1). The codon usage from … | Get A Quote |
The immunization of poultry where H5 and H7 influenza viruses (IVs) are endemic is one of the strategies to prevent unexpected zoonoses. Our group has been focused on conserved HA-epitopes as potential vaccine candidates to obtain multivalent immune responses against distinct IV subtypes. In this study, two conserved epitopes (NG-34 and CS-17) fused to flagellin were produced in a Baculovirus platform based on Trichoplusia ni larvae as living biofactories. Soluble extracts obtained from larvae expressing "flagellin-NG34/CS17 antigen" were used to immunize chickens and the efficacy of the vaccine was evaluated against a heterologous H7N1 HPAIV challenge in chickens. The flagellin-NG34/CS17 vaccine protected the ... More
