| Products/Services Used | Details | Operation |
|---|---|---|
| Codon Optimization | … The expression plasmid was designed according to the hypothetical protein sequence of TaTS (GenBank accession number WP 028494267); the TaTS DNA sequence without a stop codon (2916 bp) was back-translated and synthesized by GenScript, Inc., Piscataway, NJ, USA … | Get A Quote |
Trehalose synthase converts maltose into trehalose in a single conversion step via intramolecular transformation and is thus useful for industrial production. In this study, we synthesized a thermophilic trehalose synthase from Thermus antranikianii (TaTS), which was recombinantly expressed in Escherichia coli BL21(DE3). The recombinant TaTS showed the highest activity at pH 7.0 and 60°C, with the maximum trehalose yield (76.8%) obtained at pH 7.0 and 30°C. TaTS activity was stable over a wide pH and temperature range of 6-10 and 4-70°C, respectively, over 6 h of incubation. The enzyme activity was strongly inhibited by Co2+, Cu2+, Zn2+, sodium dodecyl sulfate, and Tris. TaTS showed a 1.48-fold higher catal... More
