| Products/Services Used | Details | Operation |
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| Codon Optimization | … For heterologous expression of Rbe1p and Rbt4p in E. coli, RBE1 and RBT4 gene sequences were codon optimized (S7 Fig), chemically synthesized with additional XhoI and BamHI restriction sites and cloned into vector pET-19b(+) (GenScript, Piscataway, USA) … | Get A Quote |
Members of the Cysteine-rich secretory protein, Antigen 5 and Pathogenesis-related 1 (CAP) protein superfamily are important virulence factors in fungi but remain poorly characterized on molecular level. Here, we investigate the cellular localization and molecular function of Rbe1p and Rbt4p, two CAP family members from the human pathogen Candida albicans. We unexpectedly found that Rbe1p localizes to budding sites of yeast cells in a disulfide bond-dependent manner. Furthermore, we show that Rbe1p and Rbt4p bind free cholesterol in vitro and export cholesteryl acetate in vivo. These findings suggest a previously undescribed role for Rbe1p in cell wall-associated processes and a possible connection between the ... More
