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The Yarrowia lipolytica PAH1 homologue contributes but is not required for triacylglycerol biosynthesis during growth on glucose.

Yeast. 2020; 
Ukey R, Carmon T, Hardman D, Hill N, Fakas S.
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Catalog Antibody … polyvinylidene difluoride membrane for immunoblot analysis Anti-Pah1 antibodies were prepared in rabbits at GenScript USA, Inc and used at a dilution of 1:7,500 The specificity of the antibodies was confirmed by immunoblotting using purified His-tagged Pah1 (GenScript Get A Quote

摘要

The PAH1-encoded phosphatidate phosphatase (PAP) catalyzes the Mg2+ -dependent dephosphorylation of phosphatidate to produce diacylglycerol, which can be acylated to form triacylglycerol (TAG). In the model oleaginous yeast Yarrowia lipolytica, TAG is the major lipid produced, and its biosynthesis requires a continuous supply of diacylglycerol, which can be provided by the PAP reaction. However, the regulation of Pah1 has not been studied in detail in Y. lipolytica, and thus its contribution to the biosynthesis of TAG in this yeast is not well understood. In this work, we examined the regulation of the PAH1-mediated PAP activity and Pah1 abundance and localization in cells growing on glucose. We found that Pah1... More

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