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Application of inorganic phosphate limitation to efficient isoprene production in Pantoea ananatis.

J Appl Microbiol. 2020; 
Nitta N, Tajima Y, Katashkina JI, Yamamoto Y, Onuki A, Rachi H, Kazieva E, Nishio Y.
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Codon Optimization … The codon-optimized nucleotide sequence of ispS (GenBank No JE994717) was chemically synthesized and cloned into pUC57 (GenScript, Piscataway, NJ, USA) to yield pUC57-ispSM A fragment of the ispS gene was PCR-amplified from … Get A Quote

摘要

Establishment of an efficient isoprene fermentation process by adopting inorganic phosphate limitation as the trigger to direct metabolic flux to the isoprene synthetic pathway.,We constructed isoprene-producing strains of Pantoea ananatis (a member of the Enterobacteriaceae family) by integrating a heterologous mevalonate pathway and a metabolic switch that senses external inorganic phosphate (Pi) levels. This metabolic switch enabled dual-phase isoprene production, where the initial cell growth phase under Pi-saturating conditions was uncoupled from the subsequent isoprene production phase under Pi-limiting conditions. In fed-batch fermentation using our best strain (SWITCH-PphoC/pIspSM) in a 1-l bioreactor, ... More

关键词

Pantoea ananatis ; fermentation; isoprene; metabolic switch; phosphate limitation