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Proteins, Expression, Isolation and Analysis | … Denatured protein samples (20 μg) were fractionated on 4–12% Bis-Tris gels (GenScript Biotech Corp, Nanjing, China) for 50 min at a constant voltage of 140 V and then electrically transferred onto a polyvinylidene fluoride (PVDF) membrane by an iBlot™ 2 Dry Blotting system … | Get A Quote |
Diabetic nephropathy (DN) is the most common cause of end-stage renal disease (ESRD). Currently, approximately 20-40% of individuals with diabetes are diagnosed with DN. Mesangial cells (MCs) are critical for maintaining and regulating glomerular filtration, and the abnormal proliferation of MCs causes the accumulation of mesangial extracellular matrix (ECM), further promoting glomerular dysfunction and renal diseases. Low molecular weight fucoidan (LMWF) extracted from Saccharina japonica could alleviate DN, but the mechanism was not analysed. Based on the ability of LMWF to ameliorate the human renal mesangial cell (HRMC) injury caused by advanced glycation end products (AGEs), we identified fibronectin (FN) ... More