| Products/Services Used | Details | Operation |
|---|---|---|
| Codon Optimization | … Expression of DES1 and DES2 in S cerevisiae The open reading frames of DES1 and DES2 were codon optimised for expression in S cerevisiae by Genscript, synthesised and supplied in the pUC-57 vector DES1 and DES2 were then excised using … | Get A Quote |
Arbuscular mycorrhizal (AM) fungi are oleaginous organisms, and the most abundant fatty acyl moiety usually found in their lipids is palmitvaccenic acid (16:1Δ11cis ). However, it is not known how this uncommon fatty acid species is made. Here, we have cloned two homologues of lepidopteran fatty acyl-coenzyme A Δ11 desaturases from the AM fungus Rhizophagus irregularis. Both enzymes, DES1 and DES2, are expressed in intraradical mycelium and can complement the unsaturated fatty acid-requiring auxotrophic growth phenotype of the Saccharomyces cerevisiae ole1Δ mutant. DES1 expression leads almost exclusively to oleic acid (18:1Δ9cis ) production, whereas DES2 expression results in the production of 16:1Δ11c... More
