Products/Services Used | Details | Operation |
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Proteins, Expression, Isolation and Analysis | 20−30 μg of whole cell lysates from the degradation assay was loaded on to 4− 12% SurePage Bis-Tris gels (GenScript, Piscataway, NJ). | Get A Quote |
Calcitonin gene-related peptide (CGRP) is a neuropeptide that is involved in the transmission of pain. Drugs targeting CGRP or a CGRP receptor are efficacious in the treatment of migraine. The canonical CGRP receptor is a complex of a G protein-coupled receptor, the calcitonin-like receptor (CLR), with an accessory protein, receptor activity-modifying protein 1 (RAMP1). A second receptor, the AMY1 receptor, a complex of the calcitonin receptor with RAMP1, is a dual high-affinity receptor for CGRP and amylin. Receptor regulatory processes, such as internalization, are crucial for controlling peptideand drug responsiveness. Given the importance of CGRP receptor activity in migraine we compared the inter... More