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| Codon Optimization | … COVID-19 campus closures: see … As an enzyme, it primarily assists in catalyzing disulfide bond formation and as a chaperon it helps in protein folding in ER lumen [18], [19] … The GC% and CA index were examined (http://www.genscript.com/cgi-bin/tools/rare_codon_analysis) … | Get A Quote |
Human interferon gamma (hIFN-γ) has an antiviral, immunoregulatory and anti-tumor properties. It is majorly used for the treatment of chronic granulomatous disease and severe malignant osteopetrosis. In the present investigation, cell level strategies were applied to address the expression machinery related problems, which are bottle neck for protein expression in Pichia pastoris. The gene encoding hIFN-γ was successfully cloned and expressed in P. pastoris (GS115) under the control of AOX promoter. About 200 μg/L of recombinant human interferon gamma (rhIFN-γ) was expressed after 1% methanol induction. The expression of rhIFN-γ was further enhanced by 2.67 fold by co-expressing the gene encoding prote... More
