| Products/Services Used | Details | Operation |
|---|---|---|
| Codon Optimization | The surface display laccase (SDL) biocatalytic cells were developed as described previously [21]. Briefly, the codon optimized LAC3 from T. versicolor was synthetized by Genscript (Piscataway, NJ). The LAC3 gene fragment was ligated into the pCTcon2 plasmid (Addgene, Cambridge, MA), yielding the plasmid pCTcon2-Lac3. E. coli TOP10 strain was used for plasmid cloning. The plasmid pCTcon2-Lac3 was transformed to the S. cerevisiae strain EBY100 to obtain SDL biocatalyst cells by using the LiAc/PEG method [51]. The control plasmid pCTcon2 (without LAC3 gene) was transformed to EBY100 to obtain the control cell without surface displayed laccase. The constructed plasmids and strains in this study are listed in Table 1. | Get A Quote |
Biocatalytic cells displaying functional enzymes on their surface have the ability to catalyze the degradation of persistent micropollutants with high enzyme accessibility and stability. However, the high migration and limited processing capability of suspended biocatalytic cells remain major challenges for practical applications in water treatment. In this study, we fabricated biocatalytic membranes (BCMs) by immobilizing biocatalytic cells, i.e., Baker's yeast with cell surface display laccase (SDL), on microporous membranes via inkjet printing and chemical crosslinking. The incorporation of SDL biocatalytic cells on the surface of the membranes was confirmed by microscopy, elemental analysis, and enzyme ass... More
