| Products/Services Used | Details | Operation |
|---|---|---|
| Gene Synthesis | The psaC–hydA2 fusion sequence (see Fig. S1, ESI†) was synthesized by Genscript (Piscataway, NJ USA) and inserted via flanking NdeI and BglII sites into the pBS-EP5.8 aadA vector digested with NdeI and BglII. T | Get A Quote |
Harnessing the power of photosynthesis to catalyze novel light-driven redox chemistry requires a way to intercept electron flow directly from the photosynthetic electron transport chain (PETC). As a proof of concept, an in vivo fusion of photosystem I (PSI) and algal hydrogenase was created by insertion of the HydA sequence into the PsaC subunit. The PSI and hydrogenase portions are co-assembled and active in vivo, effectively creating a new photosystem. Cells expressing only the PSI-hydrogenase chimera make hydrogen at high rates in a light-dependent fashion for several days. In these engineered cells, photosynthetic electron flow is directed away from CO2 fixation and towards proton reduction, demonstra... More
