| Products/Services Used | Details | Operation |
|---|---|---|
| Custom Vector Construction | … The L-RI gene (AfRI, GenBank accession number AXCX01000015.1) from A. fermentans ATCC 43279 was synthesized by Genscript (Nanjing, China), and was cloned into the recombinant vector pETDuet1-BvAI with NdeI and XhoI sites in the 5′- and 3′-termini, respectively … | Get A Quote |
L-Ribulose and L-ribose are two high-value unnatural sugars that can be biosynthesized by sugar isomerases. In this paper, an L-arabinose isomerase (BvAI) from Bacillus velezensis CICC 24777 was cloned and overexpressed in Escherichia coli BL21 (DE3) strain. The maximum activity of recombinant BvAI was observed at 45 °C and pH 8.0, in the presence of 1.0 mM Mn. Approximately 207.2 g/L L-ribulose was obtained from 300 g/L L-arabinose in 1.5 h by E. coli harboring BvAI. In addition, approximately 74.25 g/L L-ribose was produced from 300 g/L L-arabinose in 7 h by E. coli co-expressing BvAI and L-RI from Actinotalea fermentans ATCC 43279 (AfRI). This study provides a feasible approach for producing L-ribo... More
