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Proteins, Expression, Isolation and Analysis | … followed by normalization to β-actin.38 The LFLC group was set as control. The used 166 primers are listed in Table 1 (GenScript Biotech, Nanjing, China). 167 Western Blotting. Microsomal protein samples (40 μg) were loaded on a 10% 168 … | Get A Quote |
Meat-diet-induced changes in gut microbiota are often accompanied with the development of various metabolic and inflammatory disorders. The exact biochemical mechanism underlying these effects is not well elucidated. This study aims to evaluate how meat proteins in high-fat diets affect tryptophan metabolism in rats. The high-chicken-protein (HFHCH) or high-pork-protein (HFHP) diets increased levels of skatole and indole in cecal and colonic contents, feces, and subcutaneous adipose tissue. The HFHCH and HFHP diets also increased the abundance of , the Family XIII AD3011 group, and in the cecum and colon, which may be involved in the production of skatole and indole. Additionally, high-meat-protein diets induc... More