Products/Services Used | Details | Operation |
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Catalog Antibody | 02% [v/v] Brij-35) containing 200 µM CaMKKtide (Genscript), 100 µM CaCl2 , 1 µM CaM (Sigma-Aldrich, Castle Hill, NSW, Australia) 200 µM [γ-32P]-ATP (Perkin Elmer, Boston, MA, USA), 5 mM MgCl2 (Sigma-Aldrich, Castle Hill, NSW, Australia) and various concentrations of inhibitors (0–1 µM) in a standard 30 µl assay for 10 min at 30 C | Get A Quote |